Coding

Part:BBa_K1650038:Design

Designed by: Alexandra Richter   Group: iGEM15_Marburg   (2015-09-18)


GFP-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 222
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1064


Design Notes

The His-GFP enables the immobilization of GFP and GFP-Fusion-Proteins via the His-Tag.

Source

The Fusion of the His-Tag to GFP was created via PCR.

References